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KMID : 0545120080180121869
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 12 p.1869 ~ p.1873
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Identification of Psychrophile Shewanella sp. KMG427 as an Eicosapentaenoic Acid Producer
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Lee Won-Hae
Cho Ki-Woong
Park Soo-Young
Shin Kee-Sun
Lee Dong-Sun
Hwang Seon-Kap
Seo Seok-Jong
Kim Jong-Myeong
Ghim Sa-Youl
Song Bang-Ho
Lee Sang-Han
Kim Jong-Guk
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Abstract
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An isolate from holothurians was identified as an eicosapentaenoic acid (EPA)-producing bacterium KMG427, which is characterized by EPA synthesis efficiency, by thin layer and gas chromatographic analyses. The EPA production was maximized to more than 10% of the total fatty acids by incubation at 4oC after cell proliferation at 20oC. The isolated bacterium was categorized as Gramnegative, rod-shaped, aerobic, and motile with a single polar flagellum. According to phylogenetic analysis based on morphological and physiological specificities as an EPA-producing bacterium, the isolate KMG427 was found to belong to the genus Shewanella. The 16S rDNA of KMG427 was revealed to have 100% of sequence identity to that of S. hanedai CIP 103207T. Therefore, the isolate might be classified and identified as Shewanella sp. KMG427.
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KEYWORD
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Eicosapentaenoic acid, Shewanella sp. KMG427, 16S rDNA, phylogenetic tree
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